Questions

A. Describe carefully the movement of GFP-VSVP with respect to the Golgi complex.  

B. Critically compare your observations here with your expectations based on text and lecture.

C. To obtain more information about this process, cells were cooled for several hours, which retarded translocation more than translation.Consequently, newly translated GFP-VSVP accumulated in pre-Golgi regions prior to heating. Turn the page to view the results of this experiment.