QUESTIONS

1. How does Band 3 behavior in the first problem compare with what you observed in the first video in this problem set?

2. What effect, if any, does displacement of spectrin have on Band 3 behavior? How does the effect compare with what you observed when the markers were reversed, in the last experiment?

3. More Band 3 protein markers are evident at the end of the second (and third) episodes than at the beginning. Where do they come from and how do you explain their appearance?

4. How do you think the latex sphere is connected through the membrane to the underlying spectrin?