| A. Plasma Membrane Structure |
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| 1. RBC Organization -
How is the RBC membrane organized? With answers and
comments |
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| 2. General Organization
- What are the general structural features of other plasma membranes? |
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| 3. Hydropathy Data -
How are membrane protein structures inferred from hydropathy
plots? |
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| 4. Assymetry Data - What
do membrane labeling data say about membrane symmetry? |
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| 5. Gorter/Grendel Data -
What do the Gorter and Grendel data indicate about membrane
lipid organization? |
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| 6. Lipid Packing - What
do quantitative packing data indicate about membrane lipid organization? |
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| 7. Cooling Tetrahymena
- What is the effect of ambient cooling on the membrane lipid
composition of a ciliate? |
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| 8. Integral Membrane
Protein Mobility - Just how mobile are integral membrane
proteins and phospholipid in the erythrocyte plasma membrane? |
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8.a. Does a gold sphere attached to
a single Band 3 IMP move in the plane of the membrane?
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8.b. Does Band 3 movement change over
a longer period?
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8.c. Does removing the RBC cytoskeleton
(spectrin) by enzymatic digestion affect Band 3 movement?
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8.d. Does moving a Band 3 IMP affect
the organization of the underlying cytoskeleton (spectrin)?
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8.e.Does perturbing the cytoskeleton
affect Band 3 behavior?
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8.f. Does manipulating a Band 3 IMP
affect the behavior of a membrane phospholipid?
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| 9.How is the release of the
egg's cortical granules regulated? |
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| B. Plasma Membrane Function |
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| 1. Nitella - How are
solutes transported across the membrane of Nitella, a giant
alga? With answers and comments. |
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| 2. Duck RBC Osmosis -
How do duck erythrocytes regain their normal volume following
osmotic swelling? |
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| 3. RBC Ion Transport -
What mechanisms might explain the apparently anomalous ion gradients
across human, dog and cat erythrocyte membranes? |
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| 4. Ca 2+ Transport -
How does the Ca 2+ pump work? |
PDF |
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| 5. Alice's Giant Squid -
What’s the basis for membrane resting potentials in organisms
Through the Looking Glass? |
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| 6. Cl-/HCO3-
Transport - What mechanism(s) is/are responsible for RBC
chloride and bicarbonate transport? |
PDF |
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| 7. RBC Spherocytosis
- What’s the membrane basis for hereditary spherocytosis
in humans? |
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| C. Nuclear Function, Gene Replication,
and Transcription |
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| 1. Nucleoproteins - How are nucleoproteins
transported into the nucleus? |
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| D. Synthesis, Targeting, and
Sorting |
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| 1. Insect Hemoglobin
- How is insect hemaglobin synthesized? With answers
and comments |
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| 2. Membrane Proteins
- How are membrane proteins synthesized, modified and targetted? |
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| 3. IMP - What do hydropathy
data suggest about protein synthesis, modification and sorting? |
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| 4. Insulin - How is insulin
likely synthesized? With partial answers and comments |
PDF |
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5.a. PM Turnover I - How is the
plasma membrane replaced? – Abstracts of two experiments
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5.b.PM Turnover II - How is the
plasma membrane replaced – data from three experiments?
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PDF |
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| E. Cytoskeleton and Cell Motility |
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| 1. Amoeboid Locomotion
- How do amoebae move? With answers and comments. |
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| 2. Kinesin - What’s
the mechanism of kinesin’s movement along microtubules? |
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| 3. Isolated Flagella
- What is the role of the central core in flagellar movement? |
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| 4.a. Protein Movement
- How do proteins move from the ER to the Golgi complex? |
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4.b. The Temperature Shift Experiment
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4.c. Nocodazole Pretreatment
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| 5.a. Axon Movement -
What is the mechanism responsible for rapid vesicular movement
in axons? |
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5.b. Sense and Anti-Sense Axon Movement
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| F. Cell Signaling |
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| 1. Alice's Giant Squid -
How do Action Potentials work in organisms Through the Looking
Glass? |
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| 2. Tetanus Toxin - How
does tetanus toxin inhibit neurological signaling? |
PDF |
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| 3. Lophotoxin - How do
Gorgonian corals protect themselves from predation? |
PDF |
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| 4. Venus Fly Trap - How
does a Venus Fly Trap close so quickly? |
PDF |
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| 5. Nitella - What’s
the physiological and structural bases of Nitella
action potential? |
PDF |
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| 6. K+ Channels - How
do plant and animal K+ channels likely differ structurally,
based on their different transport properties? |
PDF |
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| 7. Mitogen - How do mitogens
cause erroneous growth signals? |
PDF |
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| 8. Na+/K+ Channels -
How do Na+ and K+ channels compare, and why might K+ channels
have evolved into a more varied family of proteins? |
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| G. Bioenergetics |
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| 1. Insect Flight Muscle -
How might insect flight muscle mitochondria make more ATP than
liver mitochondria? |
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| 2. Ca 2+
and Respiration - How does Ca2+ stimulate respiration in
isolated mitochondria? |
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